The other bacteria which was microscopically viewed as gram positive was missing in the incubator, another tube of sample was derived from the lab professor. The bacteria thus isolated needs to be further identified to genus and species level. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. For each biochemical test you perform, make sure to record the following in your lab book. While many traditional lab manuals are lengthy and comprehensive, descriptions of the labs in this manual are kept minimal to encourage students to further research the procedures and results on their own. You can find mutualistic bacteria living on and inside of you whom actually help you. Organization and format basic outline scientific writing can be in the form of a laboratory report, a thesis, a journal article, or some other written communication used to disseminate the results of scientific research. Pineapple research station k erala agricultural university, vazhakulam686 670, muvattupuzha. After becoming knowledgeable of microorganisms and the methods used for indication in the laboratory classroom, this study was done for the identification of an unknown bacterium. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Open the petri dish of your unknown, without placing the top of the dish down on the bench top.
The results of the exercise or experiment are what are being showcased, not the writing. The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include. There are many reasons for pinpointing the identity of microorganisms. Spring 2014 introduction the purpose of this study was multifaceted. How to identify bacillus cereus microbiology unknown lab. Help to write a hypothesis in the introduction of an. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Often in the study of microbiology, the need to identify an unknown microbe may arise. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several weeks of lab work.
This will be a vital task to take with me into my profession for many reasons. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several. View lab report microbiology lab report pdf file from mcb 3020l at university of south florida. Introductionthis study determines the workload and cost of implementing selective digestive decontamination in the microbiology laboratory, and reports the impact on microbial flora and bacterial. Antibiotics are extremely important in curing disease, but we need to be more diligent in our efforts in making sure we do not. Unknown bacteria lab report microbio biol 455 studocu. Each experiment served to help eliminated and match the unknown culture to a culture worked with in a lab. The gram positive bacteria was subjected to the following test. The labreport genre does have several sections youll need to meet in your report, which include. Microbiology lab report pdf file 1 identifying unknowns.
Clean the lab table before and after lab with the 10% bleach solution provided. General unknown reports in microbiology are written in scientific format. Oct 04, 2019 whether youre studying microbiology in high school or as a college student, youll need to write a number of lab reports. An unknown labeled as letter g was given out by the lab instructor. Example of a microbiology unknown lab paper unknown. Multiple procedures were practiced in the microbiology lab to help students better understand how bacteria thrive, grow, and regenerate.
Report for unknown identification project jessica olivares francisco alarconchaidez 12022015 negative and positive identification of unknown bacterial strains report introductionpurpose the objective of this lab was to classify the unknown bacterial strains provided utilizing a number of biochemical procedures. Create marketing content that resonates with prezi video. Celebrating prezis teacher community for teacher appreciation week. The purpose of scientific writing is not to entertain, but to inform. You will perform general biochemical tests on an unknown organism. Unknown bacteria lab report biol 307 microbiology studocu. Temperature optimum the lower the % transmittance the more turbid the broth. If you are allergic to any antibiotics please inform the instructor immediately.
Get an answer for help to write a hypothesis in the introduction of an identification of two unknown bacterias in a microbiology lab report. Student attempts identification of unknown bacteria in hopes. Bacterial identification laboratory exercise materials from. Guide to the identification of an unknown bacterium methods and report format pg. General biochemical tests microbiology resource center.
June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. Preparation of a laboratory report a lab report differs. Aug 22, 20 the general microbiology i course taught students about many kinds of bacteria. Unknown microbiology lab report for e coli free essays. Guidelines for writing a lab report for biol 215l microbiology for healthcare professionals your lab report will focus only on your unknown bacteria, which you collected, cultured, isolated, analyzed, and identified. Abstract the objective of these series of experiments was to identify an unknown bacterium. This lab should give you the background information and techniques you will need to successfully perform biochemical tests in order to identify unknown bacterial samples. Following the streak plate, a gram stain was performed using crystal violet, grams iodine, 95% ethanol, and safranin and examined. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics.
If you have an unknown bacteria and you want to identify it, youll typically perform a gram stain and then observe the colony appearance and the individual features. Jan 04, 20 microbiology unknown lab report deana issawi. If you have any questions about safe laboratory practices, ask your instructor. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as. Following the streak plate, a gram stain was performed using crystal violet, grams iodine, 95% ethanol, and safranin and examined under a microscope in order to place the unknown into either the gram positive or gram negative category lammert 2007. Unknow bacteria lab report unknown 36 introduction the purpose of this lab was to identify two unknown bacteria from a mixed culture. Preparation of a laboratory report a lab report differs from a paper in that it has defined sections. Identifying unknown bacteria using biochemical and. Example of a microbiology unknown lab report by taylor autry introduction. The lab instructor gave out a test tube labeled number 118, which consisted of two unknown bacteria, one gram negative and one gram positive. In microbiology laboratory class many techniques were studied to learn how a microorganism will react in certain conditions. Choose from 500 different sets of microbiology lab exam identification unknowns flashcards on quizlet. In this paper i will discuss the processes of how i came to find my two unknown bacteria. At the start of this semester in microbiology we have discussed the importance of being able to identify different bacteria.
Spring20 introduction in the past, it has been vital to distinguish the identities of microorganisms in the world. An unknown bacterium labeled as letter c was handed out by professor woolam. All procedures were followed as stated in the course laboratory manual and also as further elaborated on by professor woolam. This study shows the importance of identifying unknown microorganisms by applying all the methods and techniques that were studied in the microbiology course throughout the semester. The content of the report is based on parts of labs 2, 3, and 4. The procedures from the laboratory manual by mcdonald were used to accomplish the tests needed to find the two unknown bacteria 3.
First, the bacteria mix inside the unknown 109 tube was streaked across a nutrient agar plate using the quadrant streak technique. How to write microbiology unknown lab report example paper. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. Being able to culture, isolate and identify a unknown bacteria is one of the corner stones in microbiology bio 203, as well as the ability to use different techniques to distinguish and identify different bacteria.
The instructions below are designed to keep you safe in the laboratory. Bacillus subtilis unknown bacteria lab report, microbiology. Learn microbiology lab exam identification unknowns with free interactive flashcards. Module bacterial identification tests microbiology 122 notes 11 bacterial identification tests 11. Knowing their identity has aided in diagnosing numerous diseases and has discovered the most beneficial treatment. A different unknown number is given to each place bacteria are collected.
Unknown bacteria lab report introduction the purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. An unknown labeled 123 was given out by the lab instructor. These results are consistent with that test in that the unknown would need to be able to decarboxylate specific amino acids in order to neutralize its environment. Each student will hand in their own separate report even though you have performed the work together as a group.
May 24, 2016 previously, the unknown bacteria was found to utilize mixed acid type glucose fermentation, in which the unknown produces a large concentration of acid byproducts. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. A gram stain was performed in which the unknown was gram positive. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Jul 12, 2007 layman searches for pesky unknown bacteria. Pathogenicmutualistic bacteria some bacteria are pathogenic, meaning that they cause disease. Because the microorganisms used in this class are potentially harmful bl ii, no eating or drinking is allowed in the lab.
For example, your body is covered in bacteria that. At that point, you can say you have, for example, a gramnegative, aerobic streptobacilli. Because the microorganisms used in this class are potentially harmful bl. Record the unknown bacteria number in line 1 of your lab report and in the box below. The methods that were previously studied and practiced in the microbiology laboratory class were applied in order to identity two unknown bacterium. Whether youre studying microbiology in high school or as a college student, youll need to write a number of lab reports.
These methods were used to determine which two unknown bacteria were present in the test tube. In the medical field bacteria and infections of different kinds are the core of the practice. Scientific writing is written differently from other types of writing. Microbiology lab final identification of unknown bacteria. The study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. Unknown bacteria lab report microbiology paper bls. Hand in a report of the testing performed unknown identification sheet, a journal of how you arrived at the identification you indicated and a tsa plate containing the unknown organism streaked to demonstrate isolated colonies. Unknown lab report microbiology unknown a sonia kabra november 26, 2014 introduction there are numerous reasons for identifying unknown bacteria. Jun 19, 2014 example of a microbiology unknown lab report by taylor autry introduction. Start studying microbiology lab final identification of unknown bacteria flow chart. All methods that have been learned so far for identifying bacteria have been applied to this unknown. Lab manual this lab manual was created to support a microbiology course for allied health students. Bacteria lies everywhere and affects multiple people therefore knowing the causes of the bacteria can help treat an ill patient. Identification of an unknown bacterium and writing up a report.
Students then use these plates to make their own unknown plate by streaking for single colonies. Unknown bacteria are first collected by swabbing surfaces around and near the lab, then streaked on sterile lb agar plates and grown overnight in an incubator. First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment. The unknown gramnegative bacteria is enterobacter aerogenes. Bacterial identification laboratory exercise materials. Microbiology module bacterial identification tests microbiology 122 notes 11 bacterial identification tests 11. Identifying unknown bacteria lab linkedin slideshare. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. Laboratory exercises in microbiology mclaughlin and petersen iii. Microbiology bacterial unknown project linkedin slideshare. The identification is required so as to cure the illness or the.
You can then compare your sample to various known bacteria by placing it on different. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. Coming back to the trail of my grampositive organism refer to t able 2, the first test on. Unknown microbiology lab report for e coli unknow bacteria lab report unknown 36 introduction the purpose of this lab was to identify two unknown bacteria from a mixed culture. The methods that have been learned thus far for identifying bacteria have been applied to this unknown.